pMEV2HA-BRAF-K482M

Human BRAF Xpress Clones Genes of the RAF family encode three cytoplasmic serine/threonine protein kinases that are regulated by RAS and mediate cellular responses to growth signals from the cell surface to the nuclear. Human BRAF was first cloned from a testis cDNA library. Mutations of BRAF gene have been found in 66% of melanomas and many other types of cancers. All mutations reside in the kinase domain, with a single substitution, V599E accounting for 80%. K482 is a highly conserved active-site residue. BRAF, and all other kinases, mutated at this position are inactive as a protein kinase. T598 and S601 reside within kinase subdomains VII and VIII and are activating phosphorylation sites. Replacement of these two sites by acidic residues (BRAF-ED) renders BRAF constitutively active.Gene Name: Homo sapiens v-raf sarcoma viral oncogene homolog B1. - 20 ug